APPLICATION OF HPLC FOR THE ANALYSIS OF MALATHION AND S-MUSTARD MIXTURE

M, KASSEM; M., FAYED; A., Soltan; A., El Ahmad L.

doi:10.21608/iccee.2008.38445

APPLICATION OF HPLC FOR THE ANALYSIS OF MALATHION AND S-MUSTARD MIXTURE

Document Type : Original Article

Authors

¹ Egyptian Army Forces.

² Syrian Army Forces.

10.21608/iccee.2008.38445

Abstract

ABSTRACT
A method was developed for the chromatographic separation and quantification for a mixture of Malathion (as organophosphorus nerve agent), and S-mustard (as blister agent). The method used a high performance liquid chromatography (HPLC) with reversed phase C-18 column, and UV detection at 220 nm. The compounds were separated using isocratic elution acetonitrile-water (50%) at a flow rate of 0.7 mL/min in a period of 5 min. The retention times were (1.939) min for S-mustard and (3.063) min for Malathion. The calibaration curves were linear (R2 = 0.999) in the studied concentration range (200 to 1000 μg/mL). Sensitivity of the method for quantification of Malathion was found to be higher than that of S-mustard by four times. In addition, the accuracy was found to be (~ 97%) for both.

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